A technique for inoculating field sites with meloidogyne eggs.

t ion are a v o i d e d by i m m e r s i n g eggs in 0.5 % c h l o r h e x i d i n e d i ace t a t e ( H i b i t a n e ) for 15 min , fo l lowed by a wa te r r inse p r e c e d i n g i n c u b a t i o n a t 30 C. W e have n o t d e t e r m i n e d the q u a n t i t a t ive recovery of eggs f rom soil or the hatching of in fec t ive j uven i l e s in this p rocedure . F o r each e x p e r i m e n t we h a t c h be tween 100,000 a n d 500,000 eggs. U n d e r ou r condi t ions, m o r e t han 50% of the eggs co l lec ted f rom g reenhouse p o p u l a t i o n s h a t c h over a p e r i o d of 12 days, w i t h the p e a k h a t c h i n g 5-8 days af te r t r ans fe r to Z n S O , Clean , v i ab le eggs of Meloidogyne incognita were also co l lec ted by ou r m e t h o d f rom egg masses s epa ra t ed w i th 1% N a O C I (3).